Instrument: NextSeq 500
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: CD8+ T cells were isolated from LCMV-infected mice by CD8+ MACS negative selection performed on single-cell suspensions of splenocytes, followed by FACS. CD8+ T cells were isolated from B16-ova tumors by dissecting tumors from the surrounding fascia, mechanically mincing and treating with collagenase P and DNAse I for 10 minutes at 37C. Tumor-infiltrating leukocytes were enriched by CD8+ MACS positive selection (Miltenyi) and FACS isolation. In both contexts, cells were sorted directly into PBS with 2% FBS. Library construction was performed as per standard ATAC-seq protocol, as outlined in Corces et al., Nature Genetics 2016